异氟醚保护离体大鼠肺缺血再灌注损伤

摘要：Objective To study the effects of isoflurane on ischemia-reperfusion (IR)-induced injury in isolated rat lungs. Methods This study was performed on an isolated buffer-perfused rat lung model. There were four groups: CON group ( n = 6): perfusion for 60 min without ischemia; IR group (n = 6): after perfusion for 30 min, then interruption of perfusion and ventilation for 45 min followed by reperfusion for 30 min; ISO1 group (n = 6 ): 1.38% isoflurane was administered for 30 min before 45 min ischemia, then followed by 30 min reperfusion; ISO2 group (n= 6): 1.38% isoflurane was administered during the period of reperfusion. The Myeloperoxidase (MPO) activity of lung, neutrophils counts, tumor necrosis factor-α(TNF-α), superoxide dismutase (SOD) activity, malondialdehyde (MDA) in perfusate, and the Wet-to-Dry lung weight ratios were measured. Results IR caused significant increases in the wet-to-dry lung weight ratios, the activity of lung MPO, the contents of MDA and TNF-α in perfusate, but decreasess in the SOD activity and neutrophils counts. Administration of isoflurane (both ISO1 and ISO2 groups) significantly protected against IR-induced injury the via inhibiting increases of MPO activity and contents of MDA and TNF-α in perfusate. Isoflurane significantly increased the SOD activity and neutrophils counts in perfusate. No difference was found between ISO1 and ISO2 groups. Conclusion Our results suggest that isoflurane protects the lungs against IR-induced injury in isolated rat lung model.